Distribution of Insertion Sequence ISRm1 in Rhizobium meliloti and Other Gram-negative Bacteria

Characterization, nucleotide sequence, and conserved genomic locations of insertion sequence ISRm5 in Rhizobium meliloti.

A target for ISRm3 transposition in Rhizobium meliloti IZ450 is another insertion sequence element, named ISRm5. ISRm5 is 1,340 bp in length and possesses terminal inverted repeats of unequal lengths (27 and 28 bp) and contain five mismatches. An open reading frame that spans 89% of the length of one DNA strand encodes a putative transposase with significant similarity to the putative transposa...

distribution of r-factors in gram negative bacteria

a total number of 259 gram negative bacteria (entero-bacteria) isolated during the years 1973-1974 was examined. these strains were isolated from sick children referred and or hospitalized in a children hospital in tehran. the strains consisted of ; 131e.coli, 52 salmonella, 40 klebsiella, 18 shigella, 13 proteus, 3 enterobacter and 2 citrobacter. sensitivity tests were performed according to t...

Positive selection procedure for entrapment of insertion sequence elements in gram-negative bacteria.

We constructed the broad-host-range plasmid pUCD800 containing the sacB gene of Bacillus subtilis for use in the positive selection and isolation of insertion sequence (IS) elements in gram-negative bacteria. Cells containing pUCD800 do not grow on medium containing 5% sucrose unless the sacB gene is inactivated. By using pUCD800, we isolated a 1.4-kilobase putative IS element from Agrobacteriu...

Cell Envelope Structure and Functions in Gram-negative Bacteria

Antibiotic Resistance Pattern of Gram-Negative Bacteria in Gorgan

Abstract Background and Objective: The excessive use of broad-spectrum antibiotics will lead to drug resistance of microorganism and specially nosocomial organisms. Because of high incidence of antibiotic resistance in hospitals, we aimed to study antibiotic resistance to gram negative bacteria. Material and Methods: This cross-sectional study was conducted on the data of biological sampl...